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. 2016 Aug 3;2:15. doi: 10.1186/s40851-016-0050-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — In situ hybridization of NKA α1c-1 (a, b) and immunohistochemistry of NKA protein (c, d) in the kidney of eels acclimated to FW and 7 day SW. Positive mRNA signals (arrows) were localized at the proximal tubules (both FW and SW) and collecting tubules (FW > SW). From immunohistochemistry, NKA protein signals were observed at the basolateral side of proximal tubules < distal tubules < collecting tubules. Stronger immunohistochemistry signals was generally found in SW eel compared to FW eel. Top right photos show the structure of the square region in high resolution. Enclosed photos show the negative control of the hybridization incubated with sense probes. G = glomerulus; N = neck; PI = first segment of proximal tubules; PII = second segment of proximal tubules; D = distal tubules; C = collecting tubules