Fig. 1.

Effect of acute (2–10 h before isolation of muscles) metformin treatment on AMPK activation in soleus and EDL muscles from WT mice. Gray bars without and with hatches show results from saline- and metformin-treated mice, respectively. A: phosphorylated AMPK (AMPKp) in soleus muscles 2–10 h after a single dose metformin/saline. B: phosphorylated acetyl-CoA carboxylase (ACCp) in soleus muscles 2–10 h after a single dose metformin/saline. C: AMPKp in EDL muscles 2–10 h after a single dose metformin/saline. D: ACCp in EDL muscles 2–10 h after single dose metformin/saline. †Significant difference between saline and metformin treatment (main effect, P < 0.05); †P = 0.069; n = 6–8. Values are means ± SE.