Fig. 5.

Effect of chronic metformin treatment on Akt Thr³⁰⁸ and Ser⁴⁷³ phosphorylation in soleus and EDL muscles from WT and AMPK KD mice. Filled and open bars represent results from muscles incubated with or without insulin (10,000 μU/ml) respectively. A: Akt Thr³⁰⁸ phosphorylation in basal and insulin-stimulated soleus muscles from metformin- or saline-treated WT and AMPK KD mice. B: Akt Ser⁴⁷³ phosphorylation in basal and insulin-stimulated soleus muscles from metformin- or saline-treated WT and AMPK KD mice. C: Akt Thr³⁰⁸ phosphorylation in basal and insulin-stimulated EDL muscles from metformin- or saline-treated WT and AMPK KD mice. D: Akt Ser⁴⁷³ phosphorylation in basal and insulin-stimulated EDL muscles from metformin- or saline-treated WT and AMPK KD mice. *Significant difference between basal and insulin-stimulated Akt phosphorylation (main effect, P < 0.01); n = 10. Values are means ± SE.