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A: representative Western blots of AMPKp and ACCp in soleus and EDL muscles after acute (2–10 h before isolation of muscles) metformin treatment. B: representative Western blots of Aktp Thr308, Ser473, and Akt protein; AS160p Thr642, Ser711, and TBC1D4 protein; AMPKα2 protein, Rab4 protein, HKII protein, and GLUT4 protein in soleus and EDL muscles incubated without or with insulin after chronic saline or metformin treatment.
