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Protein interacting with C kinase 1 (PICK1) is expressed in rat pulmonary arterial smooth muscle cells (PASMC). A: PCR analysis of PICK1 (top: 201 bp) and β-actin (bottom: 244 bp) mRNA expression in PASMC and rat brain tissue as a positive control. B: immunoblot showing protein expression of PICK1 (∼52 kDa) in PASMC. C: immunofluorescence in PASMC showing smooth muscle 22 α (SM22α; red), PICK1 (green), and the nuclear dye SYTOX (blue).
