Fig. 4.

PICK1 does not alter ASIC1 plasma membrane (PM) localization in PASMC. Representative Western blots (A and B) and summary data (D) showing the ratio of cell surface biotinylated (A) to total (B) ASIC1 expression following 24-h treatment with the PICK1 inhibitor FSC231 (50 μM). To verify specificity of biotin labeling for cell surface proteins, a separate blot of cell surface biotinylated proteins was probed for SM22α (23kDa; C). The blot also contains a total protein sample (+) as a positive control. Values are means ± SE; n = 6/group; analyzed by unpaired t-test.