Fig. 3.

Chronic prenatal hypoxia increases bradykinin-mediated cytosolic Ca²⁺ responses. A and B: representative images (A) corresponding in time to the trace (B) of fractional fluo 4 fluorescence (F/F₀, where F is fluorescence intensity for the region of interest and F₀ is the fluorescence intensity during a period when there was no Ca²⁺ activity as determined automatically) for 4 pulmonary arterial myocytes from a fetus exposed to prenatal chronic hypoxia in the presence of extracellular Ca²⁺. Lowercase letters (a–d) denote times at which the images occurred. Colored circles in A correspond to the trace of the same color in B. Brightness of the images was adjusted for display purposes. Images were obtained with a ×40 water-immersion Plan-Apochromat objective at 1.28 frames per second. Scale bar = 20 μm.