Fig. 5.
Endothelial nitric oxide synthase (eNOS) inhibition reduces bradykinin-induced pulmonary arterial relaxation. A–D: dose-response curves of pulmonary arterial rings exposed to 10 pM–1 μM bradykinin in an additive manner normalized to %T10 μM PE for fetal sheep exposed to normoxia and prenatal chronic hypoxia and newborn sheep exposed to normoxia and prenatal chronic hypoxia in the presence of DMSO (control) for vehicle control and 100 μM nitro-l-arginine methyl ester (l-NAME) for eNOS inhibition. Lines show resultant fits to dose-response relationships with a Hill equation. Values are means ± SE. Data were analyzed by 2-way analysis of variance with a Bonferroni posttest analysis for each dose: *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.