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. 2015 Dec 23;310(7):F607–F620. doi: 10.1152/ajprenal.00169.2015

Fig. 7.

Fig. 7.

Rac1 is involved in KIM-1-mediated efferocytosis. A: HEK-293 cells were cotransfected with a construct encoding wild-type KIM-1 and either a CFP-tagged control vector (control) or CFP-tagged dominant negative Rac1 (Rac1-T17N). B: HEK-293-KIM-1 cells were treated for 4 h with vehicle (DMSO) or a Rac1 inhibitor (NSC23766; 50 μM). Cells in A and B were fed to pHrodo-labeled AC for 90 min, and the percentage of uptake was measured by flow cytometry. In A, the percentage of pHrodo Red high fluorescent cells was determined for CFP-positive cells (n = 3). **P < 0.01, ***P < 0.001 by unpaired t-test.