Fig. 4.
Effect of 1 h ergometer cycling at 65% V̇o2 peak (exercise) in noninsulin-stimulated condition at t = 30 and insulin-stimulated condition at t = 270 on the upstream (AMPK and Akt) kinases of AS160 and TBC1D1. Throughout the figure, open bars indicate a noninsulin-stimulated condition and closed bars indicate an insulin-stimulated condition. A and B: no change in AMPK Thr172 phosphorylation was detected after exercise; however, downstream ACC Ser79 phosphorylation was increased (†P < 0.05 vs. resting within noninsulin-stimulated condition). C and D: increased Akt Thr308 and Ser473 phosphorylation was observed during insulin stimulation without any exercise effect (main effect of hyperinsulinemic euglycemic clamp = §P < 0.05, #P < 0.05 vs. basal within experimental day).