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. 2016 Jun 18;5(8):1962–1972. doi: 10.1002/cam4.719

Figure 3.

Figure 3

Time course of gene expression patterns of IRF5 and LMCD1 after 7 days of mesenchymal transition (EMT) induction with TGF‐beta/OSM followed by removal of TGF‐beta/OSM for 14 days and validation of siRNA‐mediated knockdown efficiency of IRF5 and LMCD1 in H358 and MCF10a cells. (A) The relative expression levels of IRF5 and LMCD1 were measured by RT‐qPCR in H358 and (B) MCF10a cells over a 21 days EMT induction‐reversion time course in which the preEMT cells were first induced to undergo EMT via TGF‐beta/OSM‐based induction followed by removal of TGF‐beta/OSM at day 7 (*P < 0.05; **P < 0.001, as compared to preEMT cells). (C) Confirmation of knockdown efficiency of selected siRNAs on LMCD and IRF5 gene expression in H358 cells; two siRNAs were optimized and used to knockdown their relative genes at 4 nmol/L (**P < 0.001, as compared to scramble‐siR control).