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. 2016 Jun 18;5(8):1962–1972. doi: 10.1002/cam4.719

Figure 5.

Figure 5

Effects of siRNA‐mediated knockdown of IRF5 and LMCD1 on TGF‐beta/OSM‐induced epithelial mesenchymal transition (EMT) in MCF10a and A549 cells. Cells were treated with each siRNA for 48 h at indicated concentrations followed by induction of EMT using TGF‐beta/OSM for 3 days. Cells were then collected for follow‐up analysis. (A) Effects on EMT protein markers in MCF10a cells (4 nmol/L of siRNA); (B) Effects on E‐cadherin‐low‐expressing subpopulation in MCF10a cells, (4 nmol/L of siRNA); (C) Effects of siRNAs on CD133+ cell subpopulations (4 nmol/L of siRNA); (D) effects on invasion; and (E) Effects on E‐cadherin‐low‐expressing subpopulation in A549 cells (4 nmol/L and 6 nmol/L for each siRNA). Note: *P < 0.05; **P < 0.001, as compared to the postEMT scramble‐siR control.