Figure 1.

(a) The effect of OSEO on MCF-7 cells. Cell proliferation after 24 h of treatment with OSEO at indicated concentrations (50–500 μg/ml) was determined by MTT assay. (b) Propidium iodide and Hoechst 33342 staining of MCF-7 cells after 24 h treatment with OSEO (50–500 μg/ml). The control cells treated with vehicle (0.1% DMSO) and the cells treated with resveratrol (300 μg/ml) served as positive control. Fluorescence-stained apoptotic nucleic visualized at ×100 magnificence. The symbol “*” denotes that the data are statistically significant (P < 0.5) when compared to resveratrol. OSEO: Ocimum sanctum essential oil; MCF-7: Michigan cancer foundation-7