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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: FEBS J. 2015 Mar 27;282(10):2045–2059. doi: 10.1111/febs.13259

Fig. 2.

Fig. 2

PDI thiol reductase activity is inhibited by 15d-PGJ2 modification. (A) 15d-PGJ2 reduces recombinant PDI protein thiol reductase activity. Recombinant PDI (5 μM) was incubated with 1.65–33.3 μM of 15d-PGJ2 for 3 h followed by Di-E-GSSG substrate. Thiol reduction was measured by fluorescence plate reader and normalized to untreated recombinant PDI control. Data are means ± SD. n = 2–3 per group. (B) PDI activity is decreased in rat primary neuronal culture after treatment with 15d-PGJ2. Rat primary neuronal cultures were treated with 10 μM vehicle (Veh, methyl acetate) or 10 μM 15d-PGJ2 for 24 h prior to harvest. Neuronal PDI proteins were precipitated with anti-PDI antibody and a thiol reductase activity assay was performed. n = 3 per group. Data are means ± SD. **P < 0.001.