Table 1. Primer Sequences of TP53 and PTEN .
| Gene | Exon | Primer sequence 5′→3′ | Tm (°C) | Product size (bp) |
| PTEN | 1(Forward) | CAAGTCCAGAGCCATTTCCATC | 55 | 297 |
| 1(Reverse) | GCAACCTGACCAGGGTTAAATG | 55 | 297 | |
| 2(Forward) | CTCCAGCTATAGTGGGGAAAAC | 55 | 361 | |
| 2(Reverse) | GTCCATTAGGTACGGTAAGCCA′ | 55 | 361 | |
| 3(Forward) | CTACTCTAAACCCATAGAAGGG | 53 | 308 | |
| 3(Reverse) | CTTGGACTTCTTGACTTAATCGG | 53 | 308 | |
| 4(Forward) | GGGGGTGATAACAGTATCTACT′ | 53 | 285 | |
| 4(Reverse) | CAGTAAGATACAGTCTATCGGG′ | 53 | 285 | |
| 5(Forward) | CTCTGGAATCCAGTGTTTCTTT | 52 | 422 | |
| 5(Reverse) | CCAATAAATTCTCAGATCCAGG′ | 52 | 422 | |
| 6(Forward) | CTACGACCCAGTTACCATAGCA | 55 | 415 | |
| 6(Reverse) | GGCTTCTTTAGCCCAATGAGTTG | 55 | 415 | |
| 7(Forward) | GCTTGAGATCAAGATTGCAG | 50 | 439 | |
| 7(Reverse) | CAATGCCAGAGTAAGCAAAAC | 50 | 439 | |
| 8(Forward) | CAACAGATAACTCAGATTGCC | 53 | 506 | |
| 8(Reverse) | GTTCTTCATCAGCTGTACTCCT | 53 | 506 | |
| 9(Forward) | GAGGGTCATTTAAAAGGCCTCT | 53 | 458 | |
| 9(Reverse) | CTGGTAATCTGACACAATGTCC | 53 | 458 | |
| TP53 | 2-3(Forward) | TCTCATGCTGGATCCCCACT | 58 | 344 |
| 2-3(Reverse) | AGTCAGAGGACCAGGTCCTC | 58 | 344 | |
| 4(Forward) | TGAGGACCTGGTCCTCTGAC | 57 | 413 | |
| 4 (Reverse) | AGAGGAATCCCAAAGTTCCA | 57 | 413 | |
| 5-6 (Forward) | TGTTCACTTGTGCCCTGACT | 59 | 467 | |
| 5-6 (Reverse) | TTAACCCCTCCTCCCAGAGA | 59 | 467 | |
| 7(Forward) | CTTGCCACAGGTCTCCCCAA | 60 | 237 | |
| 7(Reverse) | AGGGGTCAGAGGCAAGCAGA | 60 | 237 | |
| 8-9(Forward) | TTGGGAGTAGATGGAGCCT | 59 | 455 | |
| 8-9(Reverse) | AGTGTTAGACTGGAAACTT | 59 | 455 | |
| 10 (Forward) | CAATTGTAACTTGAACCATC | 55 | 260 | |
| 10(Reverse) | GGATGAGAATGGAATCCTAT | 55 | 260 | |
| 11(Forward) | AGACCCTCTCACTCATGTGA | 59 | 245 | |
| 11(Reverse) | TGACGCACACCTATTGCAAG | 59 | 245 |