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. 2016 Mar 17;35(8):866–880. doi: 10.15252/embj.201593596

Figure EV4. USP19 negatively regulates type I IFN signaling.

Figure EV4

  • A
    Flag‐USP19‐inducible A549 cells were incubated with Doxy (200 ng/ml) overnight and infected with SeV for the indicated time points. Lysates of cells were immunoblotted with the indicated antibodies.
  • B, C
    Flag‐USP19‐inducible A549 cells were treated with Doxy (200 ng/ml) overnight. The transcription levels of IFNB and interferon‐stimulated genes (including ISG15 and ISG54) after SeV (B) or VSV (C) infection were analyzed by quantitative RT–PCR.
  • D
    Phase‐contrast (PH) and fluorescence microscopy analyses of Flag‐USP19‐inducible A549 cells with or without Doxy treatment infected with VSV‐eGFP at an MOI of 0.01 at the indicated time. Scale bar, 50 μm.
  • E
    RT–PCR analysis of gene transcription in WT and USP19 KO A549 cells after infection with VSV at the indicated time points.

Data information: Data are representative of three independent experiments, n = 3. Error bars indicate the SEM. **P < 0.01, ***P < 0.001 (two‐tailed Student's t‐test).