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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: J Med Chem. 2015 Jul 21;58(15):5770–5780. doi: 10.1021/acs.jmedchem.5b00356

Figure 5. Two conformationally-constrained diamidines (12 and 13) demonstrate decreased toxicity in cellulo.

Figure 5

(a) General synthetic schematic for conformationally-constrained compounds 11–13.

(b) Jitter plot representation of INSR splicing in cells treated with compounds 11–13. Below, representative RT-PCR gels are demonstrated, with average percentage values (± standard deviation) listed per each condition. Asterisks indicate a significant difference between the treatment concentration and mis-splicing without compound.

(c) Schematic depicting the effect of CUG repeat RNA on the splicing of TNNT2. Percentages shown indicate percentage inclusion/exclusion of alternatively spliced exon 11, as quantified in a minigene transfection system.

(d) Jitter plot representation of TNNT2 splicing in cells treated with compounds 12 and 13. Below, representative RT-PCR gels are demonstrated, with average percentage values (± standard deviation) listed per each condition. Asterisks indicate a significant difference between the treatment concentration and mis-splicing without compound.