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. 2016 Jul 25;9:409. doi: 10.1186/s13071-016-1698-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of salinomycin derivatives on the cell volume of bloodstream forms of T. brucei. Trypanosomes (5 × 10⁷ cell/ml) were incubated with 100 μM salinomycin (closed circles), SAL-E7 (open circles), SAL-AM2 (open squares), SAL-E4 (open triangles), or SAL-AM1 (open diamonds) in culture medium. Every 15 min, the absorbance at 490 nm was measured. Mean values ± SD of three experiments are shown. At the time points 30 and 45 min, the values for SAL-E7 and SAL-AM2 were statistically significantly different from the values for salinomycin (P < 0.05). At the time points 15, 30, 45 and 60 min, the values for SAL-E4 and SAL-AM1 were statistically significantly different from the values for salinomycin (P < 0.05). Note that a decrease in absorbance corresponds to an increase in cell volume