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Step Size and Rates for GlpG Unfolding/Folding from Two Linkage Methods
| Unfolding size (nm) | Unfolding rate (×10−5 s−1) | Folding rate (×10−2 s−1) | |
|---|---|---|---|
| SpyTag | 36.10 (0.05) | 8.53 (1.43) | 3.56 (0.53) |
| Disulfide | 35.63 (0.09) | 5.64 (0.91) | 3.91 (0.54) |
| P | 0.1385 | 0.0900 | 0.6442 |
Numbers in parentheses indicate standard deviation for the step size and standard error for the rate constants. The P values were assessed by Welch's t‐test.
