Figure 3.

Gene expression and genetic complementation analyses of ribonucleotide reductase small subunit (TSO2) gene copies in Arabidopsis and A. lyrata. A, Expression of the three Arabidopsis TSO2 genes in root, shoot, and flower bud. All the copies are expressed well above background. B, Genetic complementation of Arabidopsis tso2-1 with the AL.TSO2A or AL.TSO2B gene copy. Both AL.TSO2A and AL.TSO2B were predicted as expressologs by our analysis, although the sequence analysis indicated several nonsynonymous changes in the AL.TSO2B copy compared with the Arabidopsis gene (Supplemental Fig. S3A). The result of the complementation assay supported this prediction. WT, Wild type.