Figure 5.

Gene expression and genetic complementation analyses of MTP11 gene copies in Arabidopsis and A. lyrata. A, Organ expression patterns of the two A. lyrata MTP11 homologous genes assessed by RT-qPCR analysis. B, Genetic complementation of the Arabidopsis mtp11 loss-of-function mutant with AL.MTP11A and AL.MTP11B gene copies. Seedlings were grown on Murashige and Skoog medium (1% Suc, 1× Murashige and Skoog medium, and 1.2% phytoagar) for 8 d and then transferred to agarose medium supplemented with 2 mm Mn²⁺. As predicted by the expression data, both homologs could complement the mutant phenotype. WT, Wild type.