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. 2016 Jun 7;171(4):2599–2619. doi: 10.1104/pp.16.00003

Table I. List of fragment ions generated in the mass spectrum and tandem mass spectrum related to the peptide identification from the tryptic digests of ReCHS1 and ReCHS2 proteins.

Protein Retention Time Precursor Ion Charge (Ionization Mode) Theoretical Mass Experimental Mass Error Sequence Location Peptide Sequence
min m/z m/z ppm
ReCHS1 12.42 630.7983 [M+2H]+2 2 (+) 1,259.5822 1,259.5816 0.48 A44–A54 VTNSDHMTDLK
15.46 580.2929 [M+2H]+2 2 (+) 1,158.5708 1,158.5703 0.4 A96–A105 QDMVVSEVPR
16.33 828.4812 [M+H]+1 1 (+) 827.4737 827.4753 −1.94 A271–A278 DVPGLISK
17.38 847.4048 [M+3H]+3 3 (+) 2,537.1835 2,537.1964 −5.08 A125–A147 ITHVIMCTTSGVDMPGADYQLTK
1,270.600 [M+2H]+2
18.24 421.5535 [M+3H]+3 3,2 (+) 1,261.6366 1,261.6377 −0.8 A70–A79 YMHLTEDLLK
631.8258 [M+2H]+2
ReCHS2 5.61 553.2109 [M+H]+1 1,1 (+) 552.2030 552.2036 −1.09 A60–A63 MCDK
575.1909 [M+Na]+1
13.12 430.3021 [M+H]+1 1 (+) 429.2944 429.2951 −1.56 A318–A321 LGLK
13.51 607.309 [M+H]+1 1 (+) 606.3044 606.3047 −1.94 A64–A68 SMIEK
15.46 491.8245 [M+2H]+2 2,1 (+) 981.6338 981.6335 0.31 A148–A156 LLGLRPSVK
982.6379 [M+H]+1
18.55 482.2695 [M+2H]+2 2,1 (+) 962.5235 962.5185 5.13 A262–A270 LGSPFISSR
963.5290 [M+H]+1
18.96 933.9299 [M+2H]+2 2 (+) 1,864.8413 1,864.8423 −0.5 A158–A173 FMMYQQGCFAGGTVLR