Table I. List of fragment ions generated in the mass spectrum and tandem mass spectrum related to the peptide identification from the tryptic digests of ReCHS1 and ReCHS2 proteins.
Protein | Retention Time | Precursor Ion | Charge (Ionization Mode) | Theoretical Mass | Experimental Mass | Error | Sequence Location | Peptide Sequence |
---|---|---|---|---|---|---|---|---|
min | m/z | m/z | ppm | |||||
ReCHS1 | 12.42 | 630.7983 [M+2H]+2 | 2 (+) | 1,259.5822 | 1,259.5816 | 0.48 | A44–A54 | VTNSDHMTDLK |
15.46 | 580.2929 [M+2H]+2 | 2 (+) | 1,158.5708 | 1,158.5703 | 0.4 | A96–A105 | QDMVVSEVPR | |
16.33 | 828.4812 [M+H]+1 | 1 (+) | 827.4737 | 827.4753 | −1.94 | A271–A278 | DVPGLISK | |
17.38 | 847.4048 [M+3H]+3 | 3 (+) | 2,537.1835 | 2,537.1964 | −5.08 | A125–A147 | ITHVIMCTTSGVDMPGADYQLTK | |
1,270.600 [M+2H]+2 | ||||||||
18.24 | 421.5535 [M+3H]+3 | 3,2 (+) | 1,261.6366 | 1,261.6377 | −0.8 | A70–A79 | YMHLTEDLLK | |
631.8258 [M+2H]+2 | ||||||||
ReCHS2 | 5.61 | 553.2109 [M+H]+1 | 1,1 (+) | 552.2030 | 552.2036 | −1.09 | A60–A63 | MCDK |
575.1909 [M+Na]+1 | ||||||||
13.12 | 430.3021 [M+H]+1 | 1 (+) | 429.2944 | 429.2951 | −1.56 | A318–A321 | LGLK | |
13.51 | 607.309 [M+H]+1 | 1 (+) | 606.3044 | 606.3047 | −1.94 | A64–A68 | SMIEK | |
15.46 | 491.8245 [M+2H]+2 | 2,1 (+) | 981.6338 | 981.6335 | 0.31 | A148–A156 | LLGLRPSVK | |
982.6379 [M+H]+1 | ||||||||
18.55 | 482.2695 [M+2H]+2 | 2,1 (+) | 962.5235 | 962.5185 | 5.13 | A262–A270 | LGSPFISSR | |
963.5290 [M+H]+1 | ||||||||
18.96 | 933.9299 [M+2H]+2 | 2 (+) | 1,864.8413 | 1,864.8423 | −0.5 | A158–A173 | FMMYQQGCFAGGTVLR |