Table II. Steady-state kinetic parameters of purified ReCHSs with different starter units.
Results are means (n = 3) with sd values below 10% in all the cases.
Starter CoA | ReCHS1 |
ReCHS2 |
||||
---|---|---|---|---|---|---|
Km | Vmax | Efficiency (Vmax/Km) | Km | Vmax | Efficiency (Vmax/Km) | |
μm | pmol min−1 mg−1 | μm | pmol min−1 mg−1 | |||
p-Coumaroyl-CoA | 38.43 | 42 | 1.092 | 155.8 | 37.0 | 0.237 |
Acetyl-CoA | 46.6 | 3.23 | 0.069 | 37.3 | 63.5 | 1.702 |
Butyryl-CoA | 13.9 | 1.0 | 0.071 | 8.01 | 11.0 | 1.373 |
Hexanoyl-CoA | 91.65 | 1.27 | 0.013 | 11.4 | 4.7 | 0.412 |
Octanoyl CoA | 22.0 | 1.1 | 0.050 | 26.6 | 21.4 | 0.804 |