Diurnal fluctuations in crystal volume, stomatal conductance, and PSII operating efficiency of control and ABA-treated leaves. A, Diurnal fluctuations in stomatal conductance and crystal volume in pigweed leaves. Plants were grown in a growth chamber under controlled conditions (error bars represent se of mean; n = 5). B to D, Effect of ABA treatment on leaf parameters (stomatal conductance [gs] [B]; calcium oxalate crystal volume [C]; PSII operating efficiency [D]). In B to D, cut leaves remained for a time period under constant light either in artificial sap solution (control leaves, gray cycles) or in artificial sap solution containing 200 μm
ABA (ABA-treated leaves, magenta cycles). Error bars represent se of mean; n = 5. In B to D, asterisks denote significant differences between treatments (Student’s two-tailed t test; ***P < 0.001; **P < 0.01; *P < 0.05).