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. 2016 Jun 20;171(4):2826–2840. doi: 10.1104/pp.16.00342

Figure 7.

Figure 7.

Subcellular localization and photoresponse analyses of NLS-fused AsYVA. A, Subcellular localization analysis. Three-day-old dark-grown seedlings were kept in the dark or exposed to FR light (5.0 µmol m−2 s−1) for 1 h. DIC, Different interference contrast. Scale bar = 10 μm. B, Seedling de-etiolation responses of transgenic plants under dark or continuous FR light (5.0 µmol m−2 s−1). Scale bar = 5.0 mm. AsphyA:eGFP, Transgenic phyA-201 lines with eGFP-fused wild-type AsphyA; AsYVA:eGFP, transgenic phyA-201 lines with eGFP-fused Y268V-AsphyA; AsYVA:eGFP/NLS, transgenic phyA-201 lines with NLS-fused AsYVA:eGFP. C, Immunoblot analysis to show AsphyA protein levels in transgenic plants. Loading controls (AtTCTP) are shown in the bottom panel. D and E, Average hypocotyl lengths of seedlings in B. Data are the means ± sd (n ≥ 25). Means with different letters are significantly different at P < 0.01, using Duncan’s multiple range test.