Figure 1.

Putative histone chaperone OsNAPL6 is a nuclear-localized protein possessing a functional NLS near its N terminus. Nuclear and cytoplasmic protein fractions were resolved on SDS-PAGE (12%) followed either by Coomassie/silver staining (A) or immunoblotting (B) for OsNAPL6, histone H4 (a nuclear marker), and c-FBPase (a cytosolic marker). Immunoblotting for H4 and c-FBPase confirmed the purity of the nuclear and cytoplasmic fractions, respectively. C, Fluorescence micrographs show the intracellular localization of (i) GFP, (ii) OsNAPL6-GFP (OsNAPL6 fused with GFP at its C terminus), and (iii) OsNAPL6ΔNLS-GFP (NLS-deleted OsNAPL6 fused with GFP at its C terminus) in transiently transformed onion peel epidermal cells. Bar = 20 µm. See also Supplemental Figure S1.