Figure 3.

OsNAPL6 expression improves tolerance toward multiple abiotic stresses. A, Leaf strip senescence assay to analyze the response of OsNAPL6-overexpression and -knockdown toward salinity, dehydration, osmotic, and oxidative stresses. The assay was carried out to test the ability of OsNAPL6-Ox and -KD plants to resist loss in chlorophyll under salinity (200 mm NaCl), dehydration (5% PEG), osmotic (500 mm mannitol), and oxidative (5 mm H₂O₂) stress conditions. Leaf strips were floated on either water (control) or solutions containing stress agents as indicated. Numbers at the top indicate transgenic line number. Senescence was assessed until 120 h of stress. Leaf strips from wild-type, OsNAPL6-KD, and -Ox plants subjected to leaf strip senescence assay were used for estimation of either total chlorophyll (B) or chlorophyll-to-carotenoid ratio (C). FW, Fresh weight. Data shown are mean ± sd; n = 3. The * and ** represent statistically significant difference (compared to the wild type under the same conditions) at P < 0.05 and P < 0.01, respectively. Statistical significance was tested by one-way ANOVA followed by posthoc comparisons using Tukey-Kramer test.