Figure 3.

TT8 loss affects flavonoid glycosylation, jasmonic acid, and brassinosteroid biosynthesis pathways. Differentially expressed metabolites and genes, with FDR < 0.05 and absolute fold change > 2, were mapped onto the top 3 enriched pathways, namely, biosynthesis of flavonoids and their conjugation, jasmonic acid, and brassinosteroids. In the conjugation of flavonoids section, the abbreviations are as follows: K, kaempferol; Q, quercetin; G, glucoside; and R, rhamnoside. Up- and down-regulated genes and metabolites are shown in red and green, respectively. Loss of TT8 leads to coordinated increase in transcript and metabolic levels of jasmonic acid pathways, whereas the other two pathways are down-regulated. TT8 loss also affected nucleotide sugar levels along with sugar metabolism enzymes, transporters, and sugar-binding proteins. Selected pathways shown here are based on AraCyc 8.0 and cross-validated with KEGG Arabidopsis metabolic maps.