Figure 6.

TT8 directly binds to promoters of key genes. Using ChIP-PCR, enrichment of promoter fragments of target genes was quantified using real-time PCR normalized against actin2. Relative ChIP enrichment was calculated as TT8:GR (Col) DEX/TT8:GR (Col) mock. TT8:GR (Col) lines were treated with 30 µm DEX dissolved in ethanol, while mock was treated with equivalent volume of ethanol. Fold changes were calculated based on ΔΔCt values. Shown are genes associated with CAZy (A), phytohormone biosynthesis (B), and stress response (C). Results are shown as mean ± se based on three replications (P < 0.05). Direct binding of TT8 to promoter regions of these genes results in enriched expression of promoter fragments (P1–P5). The underlined enriched fragments indicate the presence of a known bHLH binding site in that region.