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. 2016 Jun 20;171(4):2810–2825. doi: 10.1104/pp.16.00469

Figure 2.

Figure 2.

SAPK2 Interacts with OsPP2C49 in vitro and in vivo. A, SAPK2 interacts with OsPP2C49 in a BiFC assay. B, SAPK2 interacts with OsPP2C49 in the in vitro pull-down assay. His-tagged SAPK2 (SAPK2-His) was used as a bait, and pull-down of OsPP2C49 was detected by anti-GST antibody. Arrows show the GST-tagged OsPP2C49 and the GST tag. C, SAPK2 interacts with OsPP2C49 in the in vivo Co-IP assay. GFP-tagged SAPK2 was immunoprecipitated using an anti-GFP antibody, and coimmunoprecipitated OsPP2C49-cMyc was detected by antic-Myc antibody. D, Scheme of the constructs used in the rice protoplast cotransfection assay. E, OsPP2C49 negatively affects the transcriptional activation activity of OsbZIP23. The fLUC/rLUC ratio represents the relative activity of 35S promoter. After transfection, half of the protoplasts were incubated with 100 μM ABA for 4 h. The values in each column are the means of three independent replicates and error bars indicate the SD. The asterisks represent significant difference determined by the Student’s t test; double asterisks indicate P < 0.01.