OsPP2C49 is a negative regulator of ABA signaling. A, Transcript levels of overexpressed OsPP2C49 and control plants. B, Decreased sensitivity of OX-OsPP2C49 plants to ABA treatment. Three-day-germinated seeds were transplanted in either half-strength MS medium (Normal) or half-strength MS medium supplemented with 2 μm ABA for 7 d. The wild-type (ZH11) and negative transgenic line (OX-OsPP2C49-13) were used as controls. C, Relative plant height of the ZH11, OX-OsPP2C49-13, and OX-OsPP2C49-32 plants under ABA treatment. The bars represent the sd (n > 6) and the double asterisks represent a significant difference determined by the Student’s t test at P < 0.01. D, OX-OsPP2C49 seedlings are shown rapidly dehydrated after exposure to air. Three-day-germinated seeds were transplanted into a rooting tube with half-strength MS medium and grown for 11 d. The sealing film was partially open to investigate the dehydration performance.