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. 2016 Jun 7;171(4):2771–2782. doi: 10.1104/pp.16.00747

Figure 4.

Figure 4.

WRKY57 directly induces the expression of JAZ1 and JAZ5. A, Relative transcript levels of JAZ1 and JAZ5. Wild-type, wrky57-1, and WRKY57OE-L10 plants were inoculated with B. cinerea. The inoculated leaves were collected at the indicated h after inoculation for RNA isolation and quantified the transcript levels of JAZ1 and JAZ5 by real-time RT-PCR. Error bars represent se of five biological replicates (n = 5). B, Promoter structure of the JAZ1 and JAZ5 genes and fragments used in the ChIP assay. W1, W2, etc. denote each W-box, numbered from left to right with sequence sites relative to the start code. Lines indicate the sequences detected by ChIP assays. C, Real-time RT-PCR data from ChIP assay with antibody against Myc with the ACTIN2 promoter (pACTIN2) as a negative control. Values are mean + se (n = 5 experiments, **P < 0.01).