Figure 5.

WRKY57 physically interacts with SIB1 and SIB2. A, Yeast-two-hybrid assays. Interaction was indicated by the ability of cells to grow on synthetic dropout medium lacking Leu/Trp/His/Ade and containing 5 mm 3-aminotriazole. The Gal4 DNA binding domain was fused with WRKY57 (shown as BD-WRKY57) and the Gal4 activation domain was fused with SIB1 or SIB2 (shown as AD-SIB1 and AD-SIB2). The Gal4 DNA binding domain expressed by pGBKT7 was used as a negative control. B, BiFC assays. Fluorescence was observed in the nuclear compartments of N. benthamiana leaf epidermal cells that resulted from complementation of the C-terminal part of YFP fused with WRKY57 (WRKY57-cYFP) and the N-terminal part of YFP fused with SIB1 or SIB2 (SIB1-nYFP and SIB2-nYFP). No signals were observed from the negative controls. BD, binding domain.