Starch, FAME, and TAG levels before and during nitrogen deprivation. Starch (A and B), FAME (C and D), and TAG (E and F) levels are reported in μg per million cells for cultures in TAP (A, C, and E) and HS (B, D, and F) media. Empty diamonds, cells cultured at 160 μmol photons m−2 s−1 (μE); empty squares, 40 μE; empty triangles, 15 μE; filled diamonds, 5 μE; filled squares, 0 μE. Error bars indicate ±sd (n = 3).