Fig 7.

(left, “MTT”) shows absorbance readings reflecting MTT metabolism (a function viable cells perform) On day 21 of differentiation cells were transfected with TNFAIP2-GFP (TNFAIP2) vector or GFP alone vector (EV) and MTT cell viability assay was performed 24 hrs later. Activated caspase 3/7 levels (right, “Casp 3/7 assay”) were measured using fluorescently labeled antibodies. Day 21 motor neurons that overexpressed TNFAIP2-GFP (TNFAIP2) compared to GFP alone (EV) were significantly less viable and have significantly more caspase 3/7 staining 24 hours after introduction of the TNFAIP2 vector. Statistical analyses were carried out using unpaired t-tests and Prism® software.