TABLE 1.
The chromatographic resolution between adjacent α2-3/α2-6 SA linkage isomers obtained from the analysis of glycopeptides and released glycans using the identical LC gradient conditions
| Peak 1 | Peak 2 | Resolution |
|
|---|---|---|---|
| Glycans | Glycopeptides | ||
| Bi-2SA(3,3) | Bi-2SA(3,6) | 4.56 | 3.80 |
| Bi-2SA(3,6) | Bi-2SA(6,6) | 4.09 | 3.67 |
| Tri-3SA(3,3,3) | Tri-3SA(3,3,6) | 3.41 | 2.30 |
| Tri-3SA(3,3,6) | Tri-3SA(3,6,6) | 3.53 | 2.79 |
| Tri-3SA(3,6,6) | Tri-3SA(6,6,6) | 4.02 | 2.75 |
| Tri-4SA(3,3,3) | Tri-4SA(3,3,6,6) | 4.09 | 2.56 |
| Tri-4SA(3,3,6,6) | Tri-4SA(3,6,6,6) | 3.49 | 2.12 |