Fig 3. Effect of conditioned medium, IGF1, and IGF1R inhibitors on motility of PDAC cells.

(A) Representative images of cell density in wound-healing assay. The number of PDAC cells migrating across the wound (broken line) was increased by conditioned medium (CM) from pancreas CAFs compared with that in control. IGF1 increased the number of migrating cancer cells. The IGF1R inhibitor picropodophyllin (PPP) and anti-IGF1R neutralizing antibody downregulated migration-stimulating activity by fibroblast CM. (B, C, D) CM from pancreas fibroblasts significantly stimulated the migratory activity of RWP-1 and MiaPaCa-2 cells, but not OCUP-AT, and Panc-1, under normoxia. Under hypoxia, the number of migrating cancer cells was significantly increased in all four cancer cell lines by CM from pancreas CAFs, and the migration-stimulating ability of CM in PDAC cells was inhibited by IGF1R inhibitors. Data are presented as mean ± SD.