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. 2016 Jun 16;8:ecurrents.md.99d88e72634387639707601b237467d7. [Version 1] doi: 10.1371/currents.md.99d88e72634387639707601b237467d7

miR31-modulation in a cell culture model of exon skipping.

Fig3

Skipped dystrophin transcripts (top), dystrophin protein (middle) and resultant protein.mRNA-1 ratio (bottom) in dystrophic myotube cultures carrying the mdx mutation (H2KSF1), either untreated (ntx), incubated with M23D skipping oligo alone (skip), or with M23D combined with miR31-modulating oligos (as indicated) added either simultaneously (T0), 24 hours after M23D addition (T24) or 48 hours after (T48). miR31-modulating oligos were added at 1:1 or 4:1 ratios with skipping oligo as indicated. Means + SEM (N=3) *=P<0.05, **=P<0.01. AU: arbitrary units. Dystrophin level normalized to vinculin (protein) or to the geometric mean of three reference genes (mRNA); see methods.