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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Sci Signal. 2016 Jul 26;9(438):ra74. doi: 10.1126/scisignal.aag0245

Fig. 6. Combination pretreatment preserves OPL morphology and retinal function in light-exposed Abca4−/−Rdh8−/− mice.

Fig. 6

Seven days after bright light exposure, cryosections were prepared from the eye cups of light-exposed Abca4−/−Rdh8−/− mice pretreated with DMSO or a combination of BRM (0.1 mg/kg bw), MTP (1 mg/kg bw), and TAM (0.05 mg/kg bw) (B + M + T). (A to C) The abundance of synaptophysin, PKCα, and calbindin D (each shown in green) was examined by immunohistochemistry. DAPI counterstaining (blue) was performed in (A) and (B) to visualize the retinal structure. White arrows in (B) and (C) denote regions of diminished staining of PKCα or calbindin D. Asterisk indicates a representative area showing diminished ONL and residual staining for synaptophysin (A) or the absence of calbindin D immunoreactivity (C). (D and E) Scotopic and photopic b-wave amplitudes were analyzed after ERG recordings were performed in Abca4−/−Rdh8−/− mice either unexposed to bright light or exposed to bright light and pretreated with either DMSO, a combination of BRM (0.1 mg/kg bw), MTP (1 mg/kg bw), and TAM (0.05 mg/kg) (B + M + T), or a combination of BRM (0.1 mg/kg bw), MTP (1 mg/kg bw), and DOX (1 mg/kg) (B + M + D). Data are means ± SD from five mice.