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. 2016 May 21;9:148–160. doi: 10.1016/j.ebiom.2016.05.025

Fig. 1.

Fig. 1

EBV infects human breast epithelial cells via CD21. (A) CD21 is expressed in immortalized normal breast epithelial cells, but not in breast cancer cells. MECs (HMLE, HMEC and MCF10A) were lysed and subjected to immunoblotting with anti-CD21 antibodies. An EBV-infected B-cell line (AKATA) served as positive control. (B) CD21 is expressed by subpopulations of MECs. (C) GFP-EBV infection of MECs. MECs were incubated with GFP-EBV for 2 h, images were taken 72 h after initial infection. (D) CD21 expression is highest in the myo-eoithelial subpopulation (CD49-high, EpCam-low). 60% of cultured primary MECs were of luminal lineage while 31% were of myoepithelial lineage. Counterstain with anti-CD21 antibodies showed significant enrichment of CD21 + cells in the myoepithelial compartment (bar graph, p < 0.05) (E) Competitive inhibition of EBV-infection of PMECs with anti-CR2 antibody HB5 (anti-CD21 moAb) and goat F(ab′)2 fragments to mouse IgG. MECs were incubated with increasing amounts HB5 followed by goat F(ab′)2 fragments (50 μg/ml)and then incubated with GFP-EBV for four hours. GFP-positive cells were counted 72 h later. Displayed are experimental triplicates ± SD. Scale bars represent 100 μm. * indicates p ≤ 0.05; ** indicates p ≤ 0.01 (two-sided T-test) (two-sided T-test).