Fig. 2.

EBV infection leads to de-differentiation and enhances proliferation of MECs. (A-D) Mammosphere cultures of EBV-infected and non-infected MECs after 2 weeks of culture. A: HMEC; B: HMEC-Ras: C: MCF-10A; D: MCF-10A-Ras. (E–H) MECs were stained with anti-CD24 anti-CD44 antibodies to visualize the stem cell compartment. E: HMEC; F: HMEC-Ras: G: MCF-10A; H: MCF-10A-Ras. (I–L) Differentiation cultures in 3D-matrigel. Mammospheres grown under stem cell conditions were transferred into differentiation medium (see Methods) and embedded in matrigel to allow for terminal differentiation. Scale bars represent 100 μm. Bar graphs display results of experimental triplicates ± SD. * indicates p ≤ 0.05.