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. 2016 May 21;9:148–160. doi: 10.1016/j.ebiom.2016.05.025

Fig. 5.

Fig. 5

Viral LMP1 expression induces expansion of the progenitor cell population and METphosphorylation. (A) Receptor tyrosine kinase signaling in EBV-infected and control MECs. Lysates from cells as indicated were analyzed using a RTK-array. A heat map was generated from the log2-transformed fluorescence intensities of phospho-proteins. (B) Immunoblotting of MET and STAT3 phosphorylation in cell lines. (C, D) Immunoblotting of MET(C) or STAT3 (D) phosphorylation in EBV- and GFP-derived tumors. (E) Extinction of LMP1 using siRNA decreases MET but not STAT3 phosphorylation. (F) Extinction of LMP1 using siRNA decreases mammosphere formation of EBV-infected MECs. (G) Inhibition of METphosphorylation with Cabozatinib (XL 184). (H, I) Treatment with Cabozatinib decreases mammosphere formation in EBV-infected, but not in control HMECs (H) or MCF10A (G). (J) Decrease of the CD24low/CD44high population in response to met-inhibitor Cabozatinib (XL 184). Bar graphs display results of experimental triplicates ± SD. Scale bars represent 100 μm. * indicates p ≤ 0.05. See also Fig. S3.