Fig. 1.

Characterization of ADAM30 expression in the brain. (a) Expression levels of ADAM30 in the brains of Alzheimer's disease cases (fifty one) and controls (forty two). All values are reported as arbitrary units (AU) following normalization against β-actin mRNA levels. All quantifications were carried out in triplicate in all individuals. The thick lines represent the median ADAM30 expression level in cases and controls. The midline represents the mean value and the upper and lower horizontal lines represent the first and third quartiles, respectively. Circles indicate individuals with extreme values (more than 2 SD above or below the mean value). p-Values refer to a Mann-Whitney non-parametric test. (b) Association of Aβx-42 and Tau loads in the brain of Alzheimer's disease cases with the expression of ADAM30 (residual correction), normalized against the expression of a-actin housekeeping gene. p values refer to Spearman's non-parametric test. (c) Immunohistochemistry experiments in human brain supporting ADAM30 expression in neurons. (d) A representative experiment measuring transfection of a shRNA vector against ADAM30 into SKNSH-5Y5Y-APP695^WT cells (ADAM30, and β-actin). (e) Mean differences (± SEM) in the amounts of sAPPα, sAPPβ, A-1β40, A-1β42 in SKNSH-Sy5Y-APP695^WT cells or (f) endogenous sAPPα, sAPPβ in SKNSH-SY5Y. Three independent experiments were performed in duplicate in SKNSH-5Y5Y-APP695^WT and in triplicate in SKNSH-SY5Y. *p < 0.05 (Mann-Whitney non-parametric test).