Fig. 5.

Impact of ADAM30 over-expression in neurons and AD-like models on Aβ secretion and CTSD activity. (a) ADAM30 expression at the protein level in adult neurons from 2 month mouse hippocampus (n = 3). (b) Mean differences (± SEM) in the secreted amounts of sAPPα, sAPPβ, and Aβ1–42 in adult neurons (Div 19) according to ADAM30^WT over-expression. Three independent experiments were performed in duplicate. *p < 0.05 (Mann-Whitney non-parametric test). (c) Differences in levels of soluble Aβ1–42 peptide concentration in the hippocampi of different transgenic mice overexpressing ADAM30^WT/^mut or not. The thick lines represent the median Aβ1–42 level. The midline represents the mean value and the upper and lower horizontal lines represent the first and third quartiles, respectively. Circles indicate individuals with extreme values (more than 2 SD above or below the mean value). p-Values refer to a Mann-Whitney non-parametric test. (d) Differences in CTSD activity in the cortex of different transgenic mice overexpressing ADAM30^WT/^mut or not. The thick lines represent the median CTSD activity level. The midline represents the mean value and the upper and lower horizontal lines represent the first and third quartiles, respectively. Circles indicate individuals with extreme values (more than 2 SD above or below the mean value). p-Values refer to a Mann-Whitney non-parametric test.