Fig. 3.

Identification of amino acids in STING required for the activation of IFN-β promoter. a–d) IFN-β promoter activities. HEK293 cells were co-transfected with pIFN-β Luc, pRG-TK and indicated plasmids. Forty-eight hours post-transfection, the reporter assay was performed. The expression levels of Renilla luciferase were used as the internal control. The experiments were performed twice, and representative figures were shown. The data were expressed as means from duplicates.