Fig. 3.

Functional analysis of neuronal network activity following compounds treatment.

a–b) Quantification of spontaneous calcium oscillations (SCO) frequency (spikes/min, a) and intensity (FAU: Fluorescence Arbitrary Units, b) in neurons treated with DMSO, fluoxetine, lithium and valproic acid (VPA). SCO were recorded over a period of 180 s, results represent mean ± SD of 5–6 biological replicates/treatment. *p < 0.01 t-test compared to DMSO treated cells. shRNA: neurons stably expressing a cDNA coding for a SHANK3 shRNA c) Representative example of recordings.