Fig. 1.

Interaction of candidate compounds by in silico calculation with PrP.

(A) Binding conformations of the 96 compounds selected by docking simulation. The red sticks represent compound conformation and the blue sticks represent the four amino acid residues reported to have a strong interaction with GN8. (B) Direct binding of compounds with PrP23–231. Sensorgrams of GN8 and NPR-056 for binding with mouse or human PrP23–231. The GN8 concentrations were 0, 0.98, 1.95, 3.91, 7.81, 15.6, 31.3 and 62.5 μM, and the NPR-056 concentrations were 0, 0.2, 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25 and 50 μM (from bottom to top). (For interpretation of the references to color in this figure legend, the reader is referred to the online version of this chapter.)