Fig. 1.

Differential gene expression and pathway analysis of RNA-seq data on ear skin from 8-week-old C57BL/6, K14E7 and K14E7/Rb^ΔL/ΔL mice. (a) Differential gene expression was detected using Empirical Bayesian statistics embedded within the Limma-voom R package where genes that pass the cut-off of Log₂-fold-change (LFC) > 2 and adjusted P < 0.01 were considered statistically significant. (b) Top 20 GO biological processes enriched in the orange intersect in K14E7 skin identified from DAVID analysis. Pathways are considered significant if the P-value (after B–H correction) is < 0.01 (orange dotted line).