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. 2016 Jun 4;9:110–119. doi: 10.1016/j.ebiom.2016.06.007

Fig. 3.

Fig. 3

Reducing MEIS1 expression in MPNST cells (S462TY) inhibits cell growth. (A) Bar graph shows that shMEIS1 (shRNA#33 or #77) reduce MEIS1 mRNA in MPNST, by qRT-PCR. (B) Western blot shows that MEIS1 protein expression is reduced by day 4 post infection (p.i.4) with both shMEIS1 viruses. Anti-β-actin was used as a loading control. (C) Line graph depicts MPNST growth after transduction with shMEIS1 or shNT control at days 1, 3 and 6 in an MTS assay (p < 0.001). (D) An immunoblot shows that MEIS1 protein levels are higher in MPNST cells than in iHSC or A549 cells. (E) Bar graph depicts the increased effects of shMEIS1 on MPNST compared to iHSC or A549. (F) Schematic shows experiment designed to test the specificity of shMEIS1, using a mouse Meis1-RFP protein and a human-targeting shMEIS1. (G) Immunoblot shows Meis1 protein expression in MPNST cells infected with Meis1-RFP OE and shMEIS1 versus vector (Empty-RFP) with shMEIS1. (H) Fluorescent (red, left) images show rescue of cells expressing Meis1-RFP OE and shMEIS1 versus Empty-RFP and shMEIS1. (I) Bar graph quantifies cell survival in MTS assays conducted day 3 or 6 after infection. (p < 0.001 via ANOVA: single factor).