Fig. 4.

Genetic deletion of Meis1 prevents formation of GEM-PNST. (A) Kaplan-Meier survival analysis shows that the lifespan of Nf1^+/−;Ink4a/Arf^−/−;Meis1^f/f;DhhCre⁺ mice is significantly increased by Meis1 deficiency (p = 0.00024; log-rank Mantel-Cox test). (B) Bar graph shows the percent of mice in each cohort that succumbed to MPNSTs versus hematopoietic neoplasms. All mice were assessed at time of death for tumors and hematopoietic neoplasms represented by enlarged liver/spleen. Mouse found dead did not show GEM-PNST. (C) Agarose gel shows that mouse sciatic nerve from Meis1-flox mice with Dhh-Cre contain cells with Meis1 deletion. The 440 bp band is the floxed Meis1 allele; the 260 bp band is the deleted Meis1 allele.